A simple, inexpensive method for the salt‐free concentration of proteins for analysis by two‐dimensional gel electrophoresis
作者:
Grace A. Maresh,
Bonnie S. Dunbar,
期刊:
ELECTROPHORESIS
(WILEY Available online 1988)
卷期:
Volume 9,
issue 1
页码: 54-57
ISSN:0173-0835
年代: 1988
DOI:10.1002/elps.1150090110
出版商: Wiley Subscription Services, Inc., A Wiley Company
数据来源: WILEY
摘要:
AbstractA rapid, inexpensive method for the salt‐free concentration of small quantities of proteins for analysis by two‐dimensional polyacrylamide gel electrophoresis (2D‐PAGE) has been developed. Proteins adsorbed to diatomaceous earth are subsequently removed using either sodium dodecyl sulfate or urea solubilization reagents for 2D‐PAGE analysis. This procedure has been found to concentrate proteins having wide ranges of molecular weight and charge. It is also valuable for the concentration of large numbers of small samples from cells culturedi
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