Automated Determination of Ammonia in Urine by Gas-Phase Molecular Absorption
作者:
PremN. Vijan,
GeorgeR. Wood,
期刊:
Analytical Letters
(Taylor Available online 1982)
卷期:
Volume 15,
issue 8
页码: 699-707
ISSN:0003-2719
年代: 1982
DOI:10.1080/00032718208069503
出版商: Taylor & Francis Group
关键词: gas phase;molecular absorption;ammonia;urine;automated
数据来源: Taylor
摘要:
A rapid determination of Ammonia in urine has been achieved. Gaseous ammonia is evolved by alkylifying urine with a saturated solution of K2CO3at 80°C followed by separation of the gas phase and measurement of characteristic absorption at 197.2 nm. The linear range of the method is between 0 and 200 μg NH3/ml. The recovery of ammonia from spiked urine samples is 106.5%. Typical results obtained on five samples from healthy subjects are presented and good agreement with a reference method is shown. Automation has been achieved by the use of a mechanical sampler, a heating bath and a peristaltic pump.
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