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New Uses for Calcium Chloride Solution as a Mounting Medium

 

作者: HerrJ. M.,  

 

期刊: Biotechnic&Histochemistry  (Taylor Available online 1992)
卷期: Volume 67, issue 1  

页码: 9-13

 

ISSN:1052-0295

 

年代: 1992

 

DOI:10.3109/10520299209109998

 

出版商: Taylor&Francis

 

关键词: calcium chloride;mounting media;lignin stain;clearing agents

 

数据来源: Taylor

 

摘要:

Fresh cross sections of stems [Psilotum nudum, Coleus blumei, andPelargonium peltatum] and roots(Setcreasea purpurea)120μm thick were fixed in FPA50(formalin: propionic acid: 50% ethanol, 5:5:90, v/v) for 24 hr and stored in 70% ethanol. The sections were transferred to water and then to 1% phloroglucin in 20% calcium chloride solution plus either hydrochloric, nitric, or lactic acid in the following ratios of phloroglucin-CaCl2solution:acid: 25:4, 20:2, or 15:5. The sections were mounted on slides either in one of the three mixtures or in fresh 20% calcium chloride solution. A rapid reaction of the acid-phloroglucin with lignin produced a deep red color in tracheary elements and an orange-red color in sclerenchyma. Fixed and stored leaf pieces fromNymphaea odoratawere autoclaved in lactic acid, washed in two changes of 95% ethanol, transferred to water, and treated with the three acid-phloroglucin-calcium chloride mixtures. The abundant astrosclereids stained an orange-red color similar to that of sclerenchyma in the sections. In addition, a new method is reported for specifically staining lignified tissues. When sections or leaf pieces are stained in aqueous 0.05% toluidine blue O, then placed in 20% calcium chloride solution, all tissues destain except those with lignified or partially lignified cell walls. Thus, toluidine blue O applied as described becomes a reliable specific test for lignin comparable to the acid-phloroglucin test.

 

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