Bacterial leaf spot of pepper,Capsicum annumL., characterized by small dark moist spots on the underside of the leaflets which eventually become brown or black and dry scabby, is reported for the first time from Pakistan. Isolations from diseased plants collected from Islamabad yielded two isolates: Xcv1and Xcv2ofXanthomonas campestrisvar.vesicatoria(Doidge) Dawson from bell pepper,C. annumvar.grossumand ornamental pepper,C. annumvar.minimumrespectively. The cultures were pathogenic in cross inoculations to both hosts and gave hypersensitive reactions in tobacco plant. The isolate Xcv1produced a copious yellow slime on yeast extract dextrose CaCO3at 25°C and 48 h incubation. These cultures were biochemically and physiologically similar.