Growth Kinetics ofClostridium bifermentansand Its Ability to Degrade TNT Using an Inexpensive Alternative Medium
作者:
Wudneh Admassu,
Anand V. Sethuraman,
Ronald Crawford,
Roger A. Korus,
期刊:
Bioremediation Journal
(Taylor Available online 1998)
卷期:
Volume 2,
issue 1
页码: 17-28
ISSN:1088-9868
年代: 1998
DOI:10.1080/10889869891214187
出版商: TAYLOR & FRANCIS
关键词: Clostridium bifermentans;TNT;corn steep liquor and molasses;Brain Heart Infusion;Tween 80;oxygen threshold.
数据来源: Taylor
摘要:
A strain ofClostridium bifermentansisolated from a munitions-supplemented anaerobic digester is known to degrade 2,4,6-trinitrotoluene (TNT) in rich media such as Brain Heart Infusion (BHI) broth. In order to make this biodegradation process commercially feasible, a new growth medium was developed. Corn steep liquor and molasses were selected as possible nitrogen and carbon sources. A medium containing 2.4% corn steep liquor and 0.4% molasses was chosen based on the value for maximum specific growth rate. The values of µmand Ksfrom continuous runs were of 0.029 min−1and 0.488 g/L, respectively. To reduce the overhead cost for maintaining an anoxic environment for this obligatory anaerobic bacterium, the threshold oxygen level under which the bacterium can survive was determined. The degradation of TNT was then carried out in a batch bioreactor, and in a continuously stirred tank bioreactor. The solubility of TNT was enhanced by using the surfactant, Tween 80, and the optimal concentration of Tween 80 was determined to be 2.5%. This concentration of Tween 80 increased TNT solubility by 100% while the effect on growth rate was minimal. Degradation of TNT was then carried out in a continuously stirred tank bioreactor and was found to follow Michaelis-Menten-type kinetics. Degradation of TNT was found to be possible under limited oxygen presence, but at a reduced degradation rate.
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