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Stability Indicating Hplc Method for the Determination of Sparfloxacin (Spar)

 

作者: A.P. Argekar,   Sunil J. Shah,  

 

期刊: Analytical Letters  (Taylor Available online 1999)
卷期: Volume 32, issue 7  

页码: 1363-1370

 

ISSN:0003-2719

 

年代: 1999

 

DOI:10.1080/00032719908542903

 

出版商: Taylor & Francis Group

 

关键词: HPLC;Sparfloxacin;Antibacterial;UV Detector

 

数据来源: Taylor

 

摘要:

A rapid, sensitive and stability indicating method for the determination of sparfloxacin (SPAR) by RP - HPLC has been developed on a Merck RP - Select B (5 μm; 12.5 cm x 4.0 mm) column using a mobile phase of water: acetonitrile: triethylamine (80 : 20 : 0.2 v/v) pH of which was adjusted to 2.6 with orthrophosphoric acid. The flow rate was 1 ml / min. and the detection was carried out at 304 nm using Waters 486 variable wavelength detector. The retention time forSPARwas 7.2 min. Linearity range was from 8 - 1000 ppm. The method showed good precision and accuracy when applied to two brands of tablets containingSPAR. In alkaline mediaSPARis stable where as it undergoes degradation in acidic and oxidising conditions generating different degradation products the nature of which is required to be established. The proposed method nicely separates the degraded products fromSPARand hence can be used as stability indicating method for the assay ofSPAR.

 

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