AbstractThe sequence of Late‐glacial and Holocene alluvial sedimentation in the middle Caquetá River Basin of Colombian Amazonia is described, based on the study of the sediments and palynology of several river bank sections and on 30 radiocarbon dates.An early Late‐glacial sedimentation cycle is recognised, followed by a minor late Late‐glacial erosion phase.The Holocene valley fill consists of grey clays (often present in the lower part of the sections) deposited in open water and silty clays often with faint yellow mottling, deposited under a regime of seasonal flooding. The base of the Holocene sections is formed by sands, where exposed. In two places the transition of sand to open‐water grey clay was dated around 10 000 yr BP and there is a suggestion that open water may have been more common at the beginning of the Holocene than later, when sedimentation by seasonal flooding became important. In many places much of the earlier Holocene sediments may have been removed by erosion and replaced by younger sediments, by a process of lateral aggradation. A considerable part of the present valley fill is younger than ca. 3500 yr. However, in several places older Holocene sediments are found, apparently only little affected by later erosion, lying below younger varzea silty clays.During the Holocene more organic sediments were formed in periods with reduced river discharge, related to drier climates in the Andes and possibly in Amazonia. These dry periods, deduced from data in the Caquetá River area, correspond well with dry phases in other parts of northwestern South America (e.g. between approximately 2700‐1900 yr BP and approximately 3200‐3800 yr BP). Rates of average net sedimentation, calculated from dated sections that apparently lack major hiatuses caused by erosion, were high in the lower Holocene, low during the middle Holocene and increase again in the upper Holocene. Levee deposits became coarser and the high river level of the Caquetá increased during the late Holocene. These phenomena may be explained by the increasing influence of man on the vegetation cover in the Andean headwater areas and possibly also in the Amazonian catchment area of the