New Applications of Bacterial Systems to Problems in Toxicology
作者:
GuengerichF. Peter,
GillamElizabeth M. J.,
ShimadaTsutomu,
期刊:
Critical Reviews in Toxicology
(Taylor Available online 1996)
卷期:
Volume 26,
issue 5
页码: 551-583
ISSN:1040-8444
年代: 1996
DOI:10.3109/10408449609037477
出版商: Taylor&Francis
关键词: bacteria;use in toxicology;cytochrome P450;glutathioneS-transferaseEscherichia coli;Salmonella typhimurium;genotoxicity assays;SOS response
数据来源: Taylor
摘要:
AbstractBacterial systems have long been of use in toxicology. In addition to providing general models of enzymes and paradigms for biochemistry and molecular biology, they have been adapted to practical genotoxicity assays. More recently, bacteria also have been used in the production of mammalian enzymes of relevance to toxicology.Escherichia colihas been used to express cytochrome P450, NADPH-cytochrome P450 reductase, flavin-containing monooxygenase, glutathioneS-transferase, quinone reductase, sulfotransferase,N-acetyltransferase, UDP-glucuronosyl transferase, and epoxide hydrolase enzymes from humans and experimental animals. The expressed enzymes have been utilized in a variety of settings, including coupling with bacterial genotoxicity assays. Another approach has involved expression of mammalian enzymes directly in bacteria for use in genotoxicity systems, particularly withSalmonella typhimurium. Applications include both the reversion mutagenesis assay and a system using a chimera with an SOS-response indicator and a reporter.
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