AbstractMeasurements were made of the small, transient offsets of vacuolar potential produced in single cells ofNitella flexilisandChara brauniiby isolated bursts of audio frequency electromagnetic radiation. The offsets increased in magnitude with decreasing frequency of the electromagnetic radiation and, below about 6 kHz, seemed to approach a lowfrequency asymptote. This frequency dependence for the offset is shown to be in accordance with a previously developed model in which the incident radiation is weakly rectified by the cell's membrane system.