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Routine Application of Polymerase Chain Reaction in the Diagnosis of Monoclonality of B‐Cell Lymphoid Proliferations

 

作者: Alberto Achille,   Aldo Scarpa,   Marina Montresor,   Maria Scardoni,   Giuseppe Zamboni,   Marco Chilosi,   Paola Capelli,   Giuseppe Franzin,   Fabio Menestrina,  

 

期刊: Diagnostic Molecular Pathology  (OVID Available online 1995)
卷期: Volume 4, issue 1  

页码: 14-24

 

ISSN:1052-9551

 

年代: 1995

 

出版商: OVID

 

关键词: Polymerase chain reaction;B-cell lymphoma;Gene rearrangement;Clonality.

 

数据来源: OVID

 

摘要:

We evaluated four polymerase chain reaction (PCR) methods for their efficiency in detecting monoclonality in a well-characterized panel of frozen and paraffin-embedded B-cell lymphoid proliferations. These approaches (referred to as FR3, FR3A, FR2, and FRI) are based on amplification of rearranged immunoglobulin heavy chain genes, using primers recognizing framework regions I, II, or III. FR3, FR3A and FR2 approaches reproducibly detected monoclonality in 51%, 72%, and 67% of DNAs from frozen lymphomas, respectively. No false-positives were observed. The combination of FR2 and FR3A methods raised the figure to 85%. Comparable results were obtained using paraffin-embedded lymphomas. Reproducibility of FRI approach was unsatisfactory. The efficiency of all PCR approaches varied depending on lymphoma type. The highest detection rate was in small/intermediate cell and the lowest in centro-follicular lymphomas. Limiting dilution assays showed that PCR methods were able to detect monoclonal B-cell DNA representing 5% of nonlymphoid and 20% of polyclonal B-cell DNA. A diagnostic protocol may include quick and cost-effective PCR screening, particularly in cases of undetermined small cell lymphoid proliferations observed in fine needle aspirates or endoscopic biopsies. This would also reduce call-up of patients to obtain unfixed biopsies.

 

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