Acetylcholine Receptor-Based Biosensor
作者:
MohyeeE. Eldefrawi,
SheblM. Sherby,
AndreasG. Andreou,
NabilA. Mansour,
Zoltan Annau,
NormanA. Blum,
JamesJ. Valdes,
期刊:
Analytical Letters
(Taylor Available online 1988)
卷期:
Volume 21,
issue 9
页码: 1665-1680
ISSN:0003-2719
年代: 1988
DOI:10.1080/00032718808066519
出版商: Taylor & Francis Group
关键词: Planar interdigitated capacitor;Receptor-based biosensor;Acetylcholine Receptor;Membrane lipids
数据来源: Taylor
摘要:
The acetylcholine (ACh) receptor protein isolated fromTorpedoelectric organ was incorporated into asolectin liposomes and immobilized noncovalently on the surface of a Planar Interdigitated Capacitive sensor to produce an ACh receptor-based biosensor. Capacitance of the biosensor, which was stable in buffered saline, increased rapidly when ACh was added. The response was dosedependent and specific for ACh, and the biosensor did not respond to six other neurotransmitters. Impure receptor preparations containing ACh esterase produced a smaller signal in response to ACh but showed enhanced response after inhibition of ACh esterase with diisopropylfluorophosphate.
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