Conceptual semi-distributed hydrological models are developed for a limited consideration of spatial heterogeneity of hydrological characteristics within a river basin. This heterogeneity can be described by area distribution functions of hydrological characteristics which can be estimated in a most effective way by a Geographical Information System (GIS). It is shown how the application of a GIS can support the development and the calibration of a conceptual hydrological model. GIS information is used to establish the criteria for sub-division of the river basin and for estimation of model structures (especially for further horizontal divisions of each basin into more homogeneous parts). That information is also used for estimation of basin characteristics and their differences between sub-basins as a support for parameter calibration by optimization. The methodology presented can be used for the development of a model structure on an objective basis and for model calibration which considers the physical explanation of model parameters. The proposed method was successfully applied to a river basin within the Mosel basin (Germany).