A powerful GABABreceptΦ‐mediated inhibition of GABAergic neurons in the arcuate nucleus
作者:
Edward Wagner,
Martha Bosch,
Martin Kelly,
K Rønnekleiv,
期刊:
NeuroReport
(OVID Available online 1998)
卷期:
Volume 9,
issue 18
页码: 4171-4177
ISSN:0959-4965
年代: 1998
出版商: OVID
关键词: Arcuate nucleus;Electrophysiology;GABA;Glutamatic acid decarboxylase;In situhybridization;Potassium conductance
数据来源: OVID
摘要:
WE combined histofluorescence within sit uhybridization to identify GABAergic neurons in the arcuate nucleus (ARC) following electrophysiological recordings, using GAD65as a marker. Intracellular recordings were made in hypothalamic slices prepared from ovariectomized guinea pigs. Over 90% of ARC neurons tested with the GABABreceptor agonist baclofen responded with a membrane hyperpolarization or an outward current. The hyperpolarization was dose-dependent, and the GABABreceptor antagonist CGP 35,348 produced a rightward shift in the agonist dose-response curve. Agonist potency was lower, and the efficacy greater, in GAD-positive neurons. The use of this novel technique for identifying GABAergic neurons thus reveals differences in the pharmacodynamics of GABABreceptor activation GABAergic and non-GABAergic ARC neurons
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