Simultaneous Determination of Plasma Enrichments of 1-13C- and15N-Labelled Phenylalanine and Tyrosine
作者:
U. Wachter,
I. Tugtekin,
M. Georgieff,
P. Radermacher,
J. Vogt,
期刊:
Isotopes in Environmental and Health Studies
(Taylor Available online 1998)
卷期:
Volume 34,
issue 3
页码: 311-316
ISSN:1025-6016
年代: 1998
DOI:10.1080/10256019808234065
出版商: Taylor & Francis Group
关键词: Carbon 13;GC/MS;nitrogen 15;phenylalanine
数据来源: Taylor
摘要:
A methylchloroformate derivative was used for the simultaneous determination of plasma enrichments of 1-13C-phenylalanine, 1-13C-tyrosine,15N-phenylalanine and15N-tyrosine by gas chromatography/mass spectrometry. All four tracer enrichments could be measured in a single GC run. A specific ion fragment was obtained for each tracer. This approach allowed an easy determination of the “tracer to tracee ratios”. Each ion fragment could be measured with an appropriate single-to-noise ratio and precision in samples obtained from 100 μl plasma. The derivatization consists of a fast one-step reaction. Therefore it is well suited for studies involving a large number of samples, such as non-steady state bolus studies.
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