Prevalence of increased intracellular signal transduction in immortalized lymphoblasts from patients with essential hypertension and normotensive subjects
作者:
Sybille Gruska,
Roland Ihrke,
Sören Stolper,
Günter Kraatz,
Winfried Siffert,
期刊:
Journal of Hypertension
(OVID Available online 1997)
卷期:
Volume 15,
issue 1
页码: 29-33
ISSN:0263-6352
年代: 1997
出版商: OVID
关键词: G protein;platelet-activating factor;calcium;sodium–proton exchange
数据来源: OVID
摘要:
ObjectiveTo determine the prevalance of enhanced signal transduction in immortalized B lymphoblasts from normotensive subjects and patients with essential hypertension.MethodsWe established Epstein-Barr virus-immortalized lymphoblast cell lines from 26 normotensive and 37 hypertensive subjects. Subsequently, we quantified rises in the cytosolic free Ca2+concentration, [Ca2+]i, evoked by 0.1 μmol/l platelet-activating factor (PAF) in Fura-2- loaded cells.ResultsPAF-induced [Ca2+]irises were independent of donor age in cells from normotensive and hypertensive subjects. Baseline values of [Ca2+]iwere not significantly different in the two groups. Using the mean + 2SD of the PAF-evoked rises in [Ca2+]iabove basal (110 nmol/l) as the upper normal value, we estimate that enhanced [Ca2+]irises are distinctly more prevalent in hypertensive subjects (27%) than they are in normotensive subjects (4%). Similarly, upon definition of normal values by the 99% confidence interval (75 nmol/l), 19% of cells from normotensive versus 43% from hypertensive subjects display enhanced intracellular signaling.ConclusionEnhanced intracellular signal transduction could be the primary defect in approximately one-third of the overall population with essential hypertension.
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