Multi‐residue enzyme immunoassays for screening for β‐agonists in faeces and feeds
作者:
Dirk Courtheyn,
Veronique Bakeroot,
Frans De Volder,
Jan Vercammen,
期刊:
Food and Agricultural Immunology
(Taylor Available online 1994)
卷期:
Volume 6,
issue 2
页码: 131-139
ISSN:0954-0105
年代: 1994
DOI:10.1080/09540109409354822
出版商: Taylor & Francis Group
关键词: Enzyme immunoassay;multi‐residue screening;β‐agonists;faeces;feeds
数据来源: Taylor
摘要:
Multi‐analyte screening methods for β‐agonists in faeces and feeds based on enzyme immunoassay (EIA) were developed. Commercially available EIA kits were used, and the results presented. The extraction for both matrices was performed under acid conditions. The addition of an organic solvent to the extradant, resulting in higher recoveries, was only retained for feeds as the background absorption for faeces increased substantially. Clean‐up for faecal extracts was achieved in a one‐step extraction with isobutyl alcohol. Detection limits in faeces were slightly different for the two types of kits used. They ranged from 0.8ng g−1for clenbuterol to 10ng g−1for terbutaline. In feeds, corresponding detection limits for both compounds were 5 ng g−1and 110 ng g−1. Confirmation of EIA positives was established by liquid chromatography with post‐column diazotation and gas chromatography‐mass spectrometry. In the screening of faeces over 1 year about 70% of the EIA positive samples were confirmed as positive.
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