Histological and Immunohistochemical Investigations of Hydroxyethyl Starch Deposits in Rat Tissues
作者:
E. Parth,
W. Jurecka,
Z. Szépfalusi,
W. Schimetta,
W. Gebhart,
O. Scheiner,
D. Kraft,
期刊:
European Surgical Research
(Karger Available online 1992)
卷期:
Volume 24,
issue 1
页码: 13-21
ISSN:0014-312X
年代: 1992
DOI:10.1159/000129184
出版商: S. Karger AG
关键词: Hydroxyethyl starch;Tissue storage;Macrophages;Immunohistochemistry
数据来源: Karger
摘要:
Tissue storage of hydroxyethyl starch (HES), a widely used artificial colloid, has been reported. In order to clarify whether storage of HES can be detected in tissues by immunohistochemical methods, use was made of a polyclonal rabbit anti-HES antiserum. Thirteen days after a single intravenous injection of HES rats were sacrificed and liver, spleen, lymph node, lung, kidney and skin were removed. On paraffin sections in all organs the anti-HES antiserum stained mainly cells which could be attributed to the mononuclear phagocyte system, as confirmed by the use of the antimacrophage monoclonal antibody ED 1. The use of a polyclonal anti-HES antiserum may allow analysis of long-term storage and possible side effects in various tissues of man.
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