Exact Tests forin SituHybridization Experiments
作者:
KeithA. Soper,
Philip Troilo,
期刊:
Journal of the American Statistical Association
(Taylor Available online 1992)
卷期:
Volume 87,
issue 417
页码: 78-83
ISSN:0162-1459
年代: 1992
DOI:10.1080/01621459.1992.10475177
出版商: Taylor & Francis Group
关键词: Cytogenetics;Experiment-wide error rate;Nonparametric test
数据来源: Taylor
摘要:
Radioactively labeled gene probes are observed as “grains” that identify specific sites of hybridization within chromosomes, suggesting potential sites for a target gene. Based on the resolution of the assay, chromosomes are subdivided into bins of approximately equal length, and the number of grains in each bin is tallied. To distinguish true hybridization sites from nonspecific binding, we propose a test statistic,Zmax, based on the number of grains in any two adjacent bins. An efficient algorithm for the exact null distribution ofZmaxis provided, as well as power calculations and a large-sample approximation. A step-down method that protects the experiment-wide error rate is given to identify multiple hybridization sites.
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