Developmental regulation of neuroligand‐induced responses in cultured oligodendroglia
作者:
Shibeshih Belachew,
Brigitte Malgrange,
Jean-Michel Rigo,
Bernard Rogister,
Paul Coucke,
Cécile Mazy-Servais,
Gustave Moonen,
期刊:
NeuroReport
(OVID Available online 1998)
卷期:
Volume 9,
issue 6
页码: 973-980
ISSN:0959-4965
年代: 1998
出版商: OVID
关键词: Development;Fluo-3;Neuropeptide;Neurotransmitter;Oligodendrocyte;Patch-clamp
数据来源: OVID
摘要:
Using whole-cell patch-clamp techniques, we show that oligosphere-derived oligodendrocyte progenitor cells (OP) display GABA-, glutamate-, 5-HT-, glycine- and acetylcholine-gated inward currents. When OP differentiate into oligodendrocytes (ODC), the amplitude of peak currents elicited by saturating concentrations of these transmitters decreases except for 5-HT. Intracellular Ca2+concentration changes induced by microperfusion of glutamate, 5-HT, TRH, met-enkephalin and substance P were monitored using a fluo-3-based calcium imaging system. When OP cells differentiate into ODC, a global decrease of the proportion of responding cells is observed. During type-2 astrocytes commitment, this proportion decreases for 5-HT, TRH- and metenkephalin stimulations whereas it remains constant for substance P and glutamate. These data demonstrate a development regulation of neurotransmitter- and neuropeptide-induced responses within the oligodendroglial lineage.
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