首页   按字顺浏览 期刊浏览 卷期浏览 Conductimetric Assay of a Bacterial Lipase, Using Triacetin as a Substrate
Conductimetric Assay of a Bacterial Lipase, Using Triacetin as a Substrate

 

作者: Catherine Ballot,   Guy Favre-bonvin,   JeanM. Wallach,  

 

期刊: Analytical Letters  （Taylor Available online 1982）
卷期: Volume 15, issue 13  

页码: 1119-1129

 

ISSN:0003-2719

 

年代: 1982

 

DOI:10.1080/00032718208064425

 

出版商: Taylor & Francis Group

 

关键词: : Lipase assay measurement;Triacetin;Electric conductivity.

 

数据来源: Taylor

 

摘要:

A conductimetric method has been applied to measure lipase activity. When using triacetin as a substrate, a linear relationship between initial rate and enzyme concentration is demonstrated up to 600 U in the cell (4 ml). Kinetic parameters of triacetin hydrolysis have been derived from conductimetric data, in the concentration range of solubility of the substrate. The limiting parameters (temperature, choice of buffer, substrate) are discussed in the last part.

 

点击下载:  PDF (275KB)



返 回