Micromethod for Determination of Thiopental in Biological Fluids by High Performance Liquid Chromatography. Application to Therapeutic Follow-Up and Pharmacokinetic Study
作者:
P. Marty,
P. Arnoux,
J.P. Cano,
期刊:
Analytical Letters
(Taylor Available online 1988)
卷期:
Volume 21,
issue 12
页码: 2241-2252
ISSN:0003-2719
年代: 1988
DOI:10.1080/00032718808059906
出版商: Taylor & Francis Group
关键词: Thiopental;High Performance Liquid Chromatography;Plasma Determination
数据来源: Taylor
摘要:
Thiopental is an ultra-short acting barbiturate, used either to induce anesthesia, or to manage intracranial hypertension associated with head injuries. A high performance liquid chromatography method vas proposed for the rapid and easy determination of thiopental in biological fluids. After precipitation of proteins with acetonitrile the chromatographic separation vas performed onto a reversed-phase column with an acetonitrile-perchlorate buffer mixture as mobile phase. Compounds were analysed at 300nm and the detection limit vas about 0.1 mg/1. The analytical procedure vas applied to the therapeutic follow-up in patients (8 childrens. 4 adults) treated with long-term infusion of thiopental after head injury, and to a pharmacokinetic study in patients receiving a single 1Y bolus of thiopental to induce anesthesia.
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