SUMMARY1 Holopedium gibberum, from twenty lakes in Rhode Island and Maine, were examined for allozyme variation at five loci to determine the pattern and degree of generic variation among sites and the genetic structure within individual lakes.2 There were significant differences in allele frequencies among sites. Most populations were fixed for a particular allele at each locus. Only five lakes had polymorphic populations.3 Polymorphic populations showed significant deviation from expected Hardy‐Weinberg genotype frequencies. In each case, there was an excess of homozygotes.4 Two lakes were examined for intra‐lake allele frequency differences. In one lake there were no differences. The other lake exhibited significant allele frequency differences between stations at the north and south ends of the lake.5 Populations were examined for the frequency distribution of composite genotypes over three loci. Most populations were dominated by one or two genotypes.6 The results suggest sporadic sexual recruitment and a high degree of genetic isolation among these populations ofH. gibberum. In these respects they resemble the permanent pond populations ofDaphnia magnaexamined by