Expression of Cystic Fibrosis Transmembrane Regulator Cl−Channels in Heart
作者:
Paul Levesque,
Páldraig Hart,
Joseph Hume,
James Kenyon,
Burton Horowitz,
期刊:
Circulation Research
(OVID Available online 1992)
卷期:
Volume 71,
issue 4
页码: 1002-1007
ISSN:0009-7330
年代: 1992
出版商: OVID
关键词: cardiac muscle;chloride channels;cyclic AMP;cystic fibrosis transmembrane regulator
数据来源: OVID
摘要:
Cyclic AMP (cAMP)-dependent chloride channels modulate changes in resting membrane potential and action potential duration in response to autonomic stimulation in heart. A growing body of evidence suggests that there are marked similarities in the properties of the cAMP-dependent chloride channels in heart and cystic fibrosis transmembrane regulator (CFTR) chloride channels found in airway epithelia or in cells expressing the CFTR gene product. We isolated poly A+mRNA from rabbit ventricle and converted it to cDNA for amplification using the polymerase chain reaction (PCR). A fragment corresponding to the nucleotide-binding domain 1 (NBD1) of the CFTR transcript was cloned. Comparison of the amino acid sequence of NBD1 of human CFTR with the deduced sequence of the rabbit heart PCR product indicated 98% identity. Northern blot analysis, using the heart amplification product as a cDNA probe, demonstrated expression of homologous transcripts in human atrium, guinea pig and rabbit ventricle, and dog pancreas.Xenopusoocytes injected with poly A+mRNA extracted from rabbit and guinea pig ventricle or dog pancreas expressed robust time-independent chloride currents in response to an elevation of cAMP. We conclude that CFTR chloride channels are expressed in heart and are responsible for the observed cAMP-dependent chloride conductance.
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