Development and Application of Highly Sensitive Anti-immune Complex ELISAs for Microcystins in Tap Water
作者:
T. Tsutsumi,
S. Nagata,
F. Yoshida,
Y. Ueno,
K.-I. Harada,
期刊:
Food and Agricultural Immunology
(Taylor Available online 2000)
卷期:
Volume 12,
issue 3
页码: 231-241
ISSN:0954-0105
年代: 2000
DOI:10.1080/09540100050140768
出版商: Taylor & Francis Group
关键词: Microcystin;Environmental Tumor Promoter;Anti-IMMUNE Complex Antibody;Sandwich Elisa;Water
数据来源: Taylor
摘要:
We developed an anti-immune complex (IC) ELISA applicable to direct determination of trace amounts of microcystins (MCs) in tap water. Comparison of two assay formats revealed that the use of anti-immune complex monoclonal antibody (MAB) in the coating step to trap anti-MC MABMC complexes improved the sensitivity as well as precision. The detection limit and quantitative range of the IC ELISA was 2 pg ml−1and 2100 pg ml−1of microcystinLR (MCLR), respectively, indicating the most sensitive of all the methods for detecting MCs reported to date. Additionally, the IC ELISA maintained good reliability through its quantitative range, as evidenced by low coefficients of variation (5.010.8 and 4.910.2% for intra- and interassay, respectively). The IC ELISA showed good cross-reactivity to microcystin-RR and microcystin-YR, suggesting major MCs found in the environment can be detected by this method. Recovery tests in which quantitative range of MCLR were added to tap water resulted in a mean recovery of 99%, with a mean standard deviation of 5.7%; therefore the IC ELISA performed well in the analysis of tap water samples. ELISA analysis of tap water samples collected in China and Japan revealed that, among 17 samples tested, two samples collected in China were positive for MCs at 4.914 pg ml−1. These results suggest that the newly developed IC ELISA can be used to monitor trace amounts of MCs in tap water.
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