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N‐Terminal Sequence of Pig Brain Choline Acetyltransferase Purified by a Rapid Procedure

 

作者: Axel Braun,   Yves‐Alain Barde,   Friedrich Lottspeich,   Werner Mewes,   Hans Thoenen,  

 

期刊: Journal of Neurochemistry  （WILEY Available online 1987）
卷期: Volume 48, issue 1  

页码: 16-21

 

ISSN:0022-3042

 

年代: 1987

 

DOI:10.1111/j.1471-4159.1987.tb13121.x

 

出版商: Blackwell Publishing Ltd

 

关键词: Choline acetyltransferase;Electroelution;Sequence;Protein purification;Affinity chromatography

 

数据来源: WILEY

 

摘要:

Abstract:A procedure is reported that allows the purification and amino terminal sequencing of pig brain choline acetyltransferase. The enzyme (present in extremely low amounts in this tissue) is eluted together with its antibody from an affinity column by a mild pH shift and the resulting enzyme‐antibody complex separated by gel electrophoresis. The band corresponding to the enzyme is electroeluted from the gel using volatile solutions allowing the direct determination of the amino acid composition and partial sequence. The first 11 residues are: Pro‐IIe‐Leu‐Glu‐Lys‐Thr‐Pro‐

 

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