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Detection of Serum Antibody by the Antimitogen Assay against Streptococcal Erythrogenic Toxins. Age Distribution in Children and the Relation to Kawasaki Disease

 

作者: YOSHIAKI ABE,   SHOKO NAKANO,   TAKAKO NAKAHARA,   YUKIO KAMEZAWA,   IWAO KATO,   HIROHARU USHIJIMA,   KAZUYA YOSHINO,   SHIGERU ITO,   SEIJI NOMA,   SHOKO OKITSU,   MASAKO TAJIMA,  

 

期刊: Pediatric Research  (OVID Available online 1990)
卷期: Volume 27, issue 1  

页码: 11-15

 

ISSN:0031-3998

 

年代: 1990

 

出版商: OVID

 

关键词: KD, Kawasaki disease;SET, streptococcal erythrogenic toxins;TdR, thymidine;Con A, concanavalin A;AMA, antimotogen assay;OD, outer diamer

 

数据来源: OVID

 

摘要:

We describe a new method to measure human serum antibody against streptococcal erythrogenic toxins that uses inhibition of lymphocyte mitogenicity of the toxins as the indicator. Sera from 53% of 53 Kawasaki disease patients contained specific inhibitory activity against A toxin, whereas only 15% had serum inhibitory activity against B toxin. The specific anti-A toxin serum inhibitor was found in 10% of 118 age-matched control patients suffering from various infections and allergic diseases (p=0.001, compared to Kawasaki disease patients). Serum inhibitory activity was detected in a small number of patients withβ-hemolytic streptococcal infection (3/19) and in none of the age-matched healthy children (0/17). However, four of seven cord blood sera samples and five of 13 sera samples from healthy neonates contained the inhibitor, a result suggesting passive transfer from mothers. Most of the antimitogen-positive sera were also positive by ELISA of IgG antibody against A toxin, and IgG fractions of the positive sera remained positive in both assays. Thus, it is possible that the specific serum inhibitor detected by the antimitogen assay represents anti-A toxin antibody. The role of toxin-producing bacteria in the pathogenesis of Kawasaki disease remains to be investigated.

 

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