The new speciesPenicillium kananaskenseis described based on an isolate from soil in aPinus contortaforest from Alberta, Canada. Colonies are characterized by their relatively rapid growth, velutinous texture, white to slightly pink conidial masses, growth at 5 °C, and absence of growth at 37 °C. The conidiophores are monoverticillate or have one metula, are up to 300 μm tall, smooth walled or with slightly roughened walls, vesiculate, and bear ampulliform phialides, 8–11.5(−14.5) × 2.5–4 μm, that produce disordered chains of ellipsoidal conidia, 3–5 × 2.5–4 μm, with smooth or finely roughened walls. The species is compared with previously described taxa and its subgeneric affinities are discussed. Preliminary data on the many secondary metabolites produced by the ex-type culture are presented.Key words:Penicillium kananaskense, soil,Pinus contortavar.latifolia, mycotoxins.