Production of Conditionally Immortalised Cell Lines from a Transgenic Mouse
作者:
Matthew C. Holley,
Patrick W. Lawlor,
期刊:
Audiology and Neurotology
(Karger Available online 1997)
卷期:
Volume 2,
issue 1-2
页码: 25-35
ISSN:1420-3030
年代: 1997
DOI:10.1159/000259227
出版商: S. Karger AG
关键词: Cochlear;Vestibular;Hair cells;Transgenic;Immortomouse;Conditional immortalisation
数据来源: Karger
摘要:
This review describes the H2kbtsA58 transgenic mouse (Immortomouse) and its application to the production of conditionally immortalised cell lines from sensory epithelia within the mammalian inner ear. Established cell lines should overcome many of the technical difficulties associated with experimental procedures in auditory and vestibular research. These include the limited amount of tissue available and the relatively complex and laborious dissection. Conditional immortalisation should also allow essential studies on the molecular and cellular mechanisms that govern both the differentiation of sensory cells and the development of sensory epithelia.
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