Corn cultures (five isolates each ofFusarium graminearumGroup 1 from wheat crowns, Group 2 from scabby wheat grains and from ear rot of corn and five isolates ofF. crookwellense)were screened for their ability to produce deoxynivalenol (DON), nivalenol (NIV), fusarenon‐x (FUS‐X) and zearalenone (ZEA). Nine of the tenF. graminearumisolates from wheat produced DON (5–165 μg g−1) but none produced either NIV or FUS‐X. Conversely, 3/5 and 2/5 of theF. graminearumisolates from corn produced NIV (5–40 μg g−1) and FUS‐X (5–7/ μg g−1), respectively, while none produced DON. All but one of theF. graminearumisolates produced ZEA (2–1160 μg g−1). None of theF. crookwellenseisolates produced DON, but 5/5 and 4/5 produced NIV (6–170 μg g−1) and FUS‐X (3–90 μg g−1), respectively, and all produced ZEA (605–1030 μg g−1). The results confirmed previous findings on the presence of two distinctF. graminearumchemotypes.