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Diminution of CD4 surface protein but not CD4 messenger RNA levels in monocytic cells infected by HIV‐1

 

作者: Romas Geleziunas,   Stephan Bour,   François Boulerice,   John Hiscott,   Mark Wainberg,  

 

期刊: AIDS  (OVID Available online 1991)
卷期: Volume 5, issue 1  

页码: 29-34

 

ISSN:0269-9370

 

年代: 1991

 

出版商: OVID

 

关键词: CD4 protein;CD4 mRNA;monocytes;HIV-1

 

数据来源: OVID

 

摘要:

As expected, the productive infection of several monocytic cell lines by HIV-1 led to a diminution of cell-surface CD4 antigen. However, unlike findings reported for HIV-1-infected T cells, this decrease was not accompanied by a similar reduction in levels of CD4 transcripts. OKT4 monoclonal antibodies (MAbs) to CD4 were used in immmunoprecipitation experiments to show that intracellular CD4 levels were diminished in U-937 monocytic cells that had been infected by HIV-1. These MAbs also coprecipitated viral gp120, indicating that CD4-gp120 complexes are present in infected monocytes. Our results therefore demonstrate that cell-surface down-modulation of CD4 is exclusively a post-transcriptional event in HIV-1-infected monocytic cells. These data suggest that HIV-1-mediated depletion of cell-surface CD4 in monocytes does not involve transcript down-modulation as has been reported in T lymphocytes.

 

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