Impaired Interleukin-3 mRNA Decay in Autocrine Mast Cell Tumors after Transient Calcium Ionophore Stimulation
作者:
HirschHans H.,
BackenstossVerena,
MoroniChristoph,
期刊:
Growth Factors
(Taylor Available online 1996)
卷期:
Volume 13,
issue 1-2
页码: 99-110
ISSN:0897-7194
年代: 1996
DOI:10.3109/08977199609034570
出版商: Taylor&Francis
关键词: mRNA stability;calcium ionophores;cycloheximide;puromycin;actinomycin-D
数据来源: Taylor
摘要:
AbstractAutocrine interleukin-3 (IL-3) expression of v-H-ras transformed mast cell tumors involves either IL-3 mRNA stabilization (class-I tumors) or enhanced IL-3 transcription (class-II tumors). Since calcium ionophores induce IL-3 expression in untransformed PB-3c cells by transcript stabilization, we asked whether class-I tumor could still respond to calcium ionophores. We found that ionomycin treatment further increased IL-3 mRNA expression of class-I tumor cells. Following ionomycin wash-out, IL-3 mRNA decay was slower in class-I tumor cells than in class-II tumor or precursor cell lines (t1,2>50 min versus<20 min, respectively). Somatic cell fusion of the class-I tumor cells with the non-tumorigenic PB-3c cells resulted in reversion to rapid decay after ionomycin wash-out. The data indicate that a recessive defect of IL-3 mRNA degradation can be revealed in class-I tumor cells by transient calcium ionophore stimulation. However, IL-3 mRNA stabilization operating constitutively in class-I tumor cells appears to be distinct from the ionomycin induced process.
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