Characterization of an unusual cAMP receptor and its related polypeptides inDictyostelium discoideum
作者:
Adrian Tsang,
Caroline Grant,
Carolyn Kay,
Gerard Bain,
Michael Greenwood,
Toshiaki Noce,
Masao Tasaka,
期刊:
Developmental Genetics
(WILEY Available online 1988)
卷期:
Volume 9,
issue 4‐5
页码: 237-245
ISSN:0192-253X
年代: 1988
DOI:10.1002/dvg.1020090406
出版商: Wiley Subscription Services, Inc., A Wiley Company
关键词: gene regulation;immunoblotting;rapid‐developing variants;molecular cloning
数据来源: WILEY
摘要:
AbstractSeveral lines of evidence indicate that cAMP modulates developmental gene activity via cell‐surface receptors. We describe here a novel cAMP receptor, CABP1, whose properties are consistent with the idea that this protein is involved in gene regulation. Firstly, immunological techniques using anti‐CABP1 antibodies as probes showed that this cAMP receptor can be detected on the surface of developing cells. Secondly, there is a steady migration of CABP1 to the nucleus during development. Thirdly, some genetic variants exhibiting an altered pattern of development are found to possess modified CABP1. We also showed that CABP1 co‐purifies with at least seven other polypeptides which share common epitopes with CABP1. Interestingly, four of the CABP1‐related polypeptides can be detected on the cell surface as well as in the
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