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Activation of Mouse Oocytes, Fertilization and Development of Mouse Embryos in Vitro after Staining with Trypan Blue or Fluorescein Diacetate

 

作者: NijsM.,   BlerkM. Van,   Van SteirteghemA. C.,  

 

期刊: Biotechnic&Histochemistry  （Taylor Available online 1992）
卷期: Volume 67, issue 6  

页码: 351-355

 

ISSN:1052-0295

 

年代: 1992

 

DOI:10.3109/10520299209110048

 

出版商: Taylor&Francis

 

关键词: fluorescein diacetate;trypan blue;in vitro fertilization;in vitro development;mouse oocyte;mouse embryo

 

数据来源: Taylor

 

摘要:

The influence of vital staining with trypan blue or fluorescein diacetate on the fertilization of mouse oocytes and the developmental potential of mouse embryos was assessed. Neither stain induced spontaneous activation in mouse oocytes, nor did they impair the in vitro development and implantation of mouse zygotes, two-cell embryos, stressed morulae or blastocysts. However, fertilization and subsequent development of mouse oocytes have been shown to be reduced by vital staining.

 

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