Single L-Type Calcium Channels in Smooth Muscle Cells From Resistance Arteries of Spontaneously Hypertensive Rats
作者:
Yusuke Ohya,
Takuya Tsuchihashi,
Shuntaro Kagiyama,
Isao Abe,
Masatoshi Fujishima,
期刊:
Hypertension
(OVID Available online 1998)
卷期:
Volume 31,
issue 5
页码: 1125-1129
ISSN:0194-911X
年代: 1998
出版商: OVID
数据来源: OVID
摘要:
The amplitude of the whole-cell L-type Ca2+channel current recorded from vascular smooth muscle cells is reportedly greater in spontaneously hypertensive rats (SHR) than in Wistar-Kyoto rats (WKY). However, no study has examined properties of single Ca2+channels in arterial cells from these strains. To further test the hypothesis that activation of L-type Ca2+channels in arterial smooth muscle cells would be enhanced in SHR, we recorded single Ca2+channel currents in resistance mesenteric artery cells from SHR and WKY (8 to 9 weeks of age) using a cell-attached patch clamp technique. With 50 mmol/L Ba2+in the recording pipette, the depolarizing pulse from a holding potential of -40 mV evoked the single L-type Ca2+channel current. Opening of the single channels was more frequent in cells from SHR than from WKY. Single-channel conductance (20 pS) and open time (1 ms at 0 mV) did not differ in the two strains. The results suggest that an increased amplitude of the whole-cell current can be attributed to the enhanced opening of single Ca2+channels in the arterial smooth muscle cells from SHR compared with WKY. (Hypertension. 1998;31:1125-1129.)
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