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Quantitative Analysis of DNA Gel Electrophoresis Patterns by Pulsed Laser Induced Fluorescence

 

作者: Joshua B. Halpern,   Jokoo Quaye,   Fabienne Newsome,   Marian Johnson-Thompson,  

 

期刊: Instrumentation Science & Technology  （Taylor Available online 1990）
卷期: Volume 19, issue 1  

页码: 15-28

 

ISSN:1073-9149

 

年代: 1990

 

DOI:10.1080/10739149008543715

 

出版商: Taylor & Francis Group

 

数据来源: Taylor

 

摘要:

A pulsed laser excited fluorescence detector is described for the quantitative analysis of DNA and RNA components separated by electrophoresis. The system is based on measurement of the fluorescence from ethidium bromide complexed with DNA where the fluorescence is excited by a nitrogen laser. In principal the method should work for any fluorescing stain.

 

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