Somatic chromosomes of 15 roses: diploid, triploid, tetraploid and pentaploid were studied. The chromosomes were grouped into types based on their relative lengths and arm ratios. The longest chromosome of a complement was found to be twice the length of small chromosomes. Two small chromosomes possessed nucleolar organizing regions both in diploids and tetraploids. Additional evidence was obtained from the PMCs where one bivalent was found attached to the nucleolus. There were a few minor differences in the karyotypes of theRosaspecies, hybrids and cultivars, which could be attributed to the hybridization and prolonged hystory of cultivation of roses.