Continuous Infusion of Growth Hormone-Releasing Factor: Effects on Pulsatile Growth Hormone Secretion in Normal Rats
作者:
William B. Wehrenberg,
期刊:
Neuroendocrinology
(Karger Available online 1986)
卷期:
Volume 43,
issue 3
页码: 391-396
ISSN:0028-3835
年代: 1986
DOI:10.1159/000124554
出版商: S. Karger AG
关键词: Growth hormone-releasing factor;Growth hormone;Rats
数据来源: Karger
摘要:
Theeffects of a continuous infusion of growth hormone-releasing factor (GRF) on pulsatile growth hormone (GH) secretion were studied in conscious, freely moving rats. Male Sprague-Dawley rats were prepared with 2 indwelling venous catheters and were then housed individually for 6–16 days in isolation chambers. After this period, normal saline, 1.5 µg/h GRF or 15 µg/h GRF were continuously infused for 8 or 31 h via one of the catheters. Blood samples were drawn at ≈ 20-min intervals via the other catheter during the last 6–7 h of the infusion. Plasma GH concentrations were determined by RIA. Saline-treated animals exhibited the typical pattern of spontaneous GH pulses. The frequency of GH pulses in animals infused with either dose of GRF was not different from that of the saline-infused rats. Likewise, trough GH concentrations were similar in all three treatment groups. However, peak GH concentrations in the rats receiving the 15-µg/h GRF infusion were remarkably higher than the concentrations observed in the other two groups, regardless of the duration of the infusion. This resulted in mean GH concentrations being significantly higher (p
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