Silver staining of DNA in polyacrylamide gels: Linearity and effect of fragment size
作者:
David Goldman,
Carl R. Merril,
期刊:
ELECTROPHORESIS
(WILEY Available online 1982)
卷期:
Volume 3,
issue 1
页码: 24-26
ISSN:0173-0835
年代: 1982
DOI:10.1002/elps.1150030105
出版商: Wiley Subscription Services, Inc., A Wiley Company
数据来源: WILEY
摘要:
AbstractA simplified, inexpensive silver stain for proteins in polyacrylamide gels also stains DNA with a sensitivity of 1 ng (<0.03 ng/mm2). Sensitivity of detection is superior to ethidium bromide and, for 11 double‐stranded fragments of ϕX174 DNA, staining is linear with respect to concentration. Surprisingly, the slope of staining is similar for pieces of DNA larger than 300 base pairs, but is greater for fragments of smaller size. Staining of DNA with silver is a practical alternative to visualization with ethidium bromide and may serve as a tool for elucidating the basis of silver staini
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