A Two Step Stain for Normal and Leukemic Monocytes Using Two Different Dyes Applied in Sequence
作者:
KassLawrence,
期刊:
Biotechnic&Histochemistry
(Taylor Available online 1992)
卷期:
Volume 67,
issue 2
页码: 68-72
ISSN:1052-0295
年代: 1992
DOI:10.3109/10520299209110010
出版商: Taylor&Francis
关键词: monocytes;monocytic leukemia;basic blue 54;basic blue 141
数据来源: Taylor
摘要:
A staining procedure for monocytes in specimens of blood and bone marrow was developed. The technique was a two step procedure in which unfixed cells were exposed first to a methanolic solution of C.I. basic blue 54. Next, an aqueous alkaline buffered solution of C.I. basic blue 141 was added to the first staining solution. After staining for 10 min in the solution with two stains, slides or coverslips were washed for 5 sec in pH 5.6 phosphate buffer and drained dry. The cytoplasm of monocytes stained intensely deep purple and frequently nuclei were stained red. Similar staining was not found in other types of normal or abnormal blood and bone marrow cells.
点击下载:
PDF (480KB)
返 回