AbstractThe thermoelectrogravitational electrophoresis column without reservoirs has been conceived following the principle of the Clusius‐Dickel thermal diffusion column. The transport equation approach in thermal diffusion developed by Jones and Furry to explain the behavior of a conventional thermogravitational thermal diffusion column has been applied to describe the electrophoretic separation in the thermoelectrogravitational electrophoresis column. Equations have been established for a system in which one single or one hypothetically single component is mobile and the other species are at their isoelectric points.Theoretical calculations for the velocity profiles, temperature distribution, and the steady state batch separation factors have been made with field strength, temperature difference, membrane spacing, and electric mobility as variables, and expected trends of the results are discussed using the steady state solution of the transport equation for batch operatio