Spectrophotometric Determination of B6Vitamins by Coupling with DiazotizedP-Sulfanilic Acid in Micellar Medium ofN-Cetylpyridinium Chloride
作者:
Llorenç Monferrer-Pons,
Lluis Alvarez-Rodríguez,
Josep Esteve-Romero,
M. Celia García-Alvarez-Coque,
期刊:
Analytical Letters
(Taylor Available online 1999)
卷期:
Volume 32,
issue 1
页码: 51-63
ISSN:0003-2719
年代: 1999
DOI:10.1080/00032719908542598
出版商: Taylor & Francis Group
关键词: Spectrophotometry;B6;vitamins;Pharmaceuticals;Diazotizedp-sulfanilic acid;N-Cetylpyridinium chloride;Micellar medium
数据来源: Taylor
摘要:
The water-soluble B6vitamins pyridoxine, pyridoxal and pyridoxamine were determined by coupling with the diazonium ion ofp-sulfanilic acid, in a micellar medium ofN-cetylpyridinium chloride (NCPC), with spectrophotometric measurement in the visible region (450 nm). A stable azo dye, with increased sensitivity with respect to the derivative obtained in non-micellar medium, was rapidly formed. The addition of toxic mercury(II) acetate, recommended in a previous procedure to develop the colour for pyridoxine and pyridoxamine, was not required in the presence of NCPC. The procedure was applied to the determination of pyridoxine hydrochloride in commercial pharmaceutical preparations, with recoveries between 94 and 107%, and repeatabilities below 1%. The differences in the coupling reaction rates of the B6vitamins, in the presence of boric acid and NCPC micellar medium, permitted the differential-kinetic analysis of binary mixtures of the three B6vitamins.
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