A method for the in vitro determination of feline corneal endothelial permeability
作者:
McDermottMark L.,
WatskyMitchell A.,
GeroskiDayle H.,
EdelhauserHenry F.,
期刊:
Current Eye Research
(Taylor Available online 1990)
卷期:
Volume 9,
issue 12
页码: 1129-1136
ISSN:0271-3683
年代: 1990
DOI:10.3109/02713689009003468
出版商: Taylor&Francis
数据来源: Taylor
摘要:
A mounting block for in vitro perfusion of the cat cornea is described. Using this apparatus and the techniques of Araie, the permeability (Pac) of the normal cat corneal endothelium to carboxyfluorscein was determined to be 2.5 plusmn; 0.2×10-4cm/min. To assess the sensitivity of this technique in determining changes in Pacassociated with alterations in endothelial, morphology, three cats underwent 2 successive unilateral, central, 10 mm diameter circular areas of endothelial debridement 6 weeks apart. Six weeks following the second wounding all 3 animals underwent morphometric analysis and Pacdetermination. A trend toward an elevation in Pacwith extreme reductions in cell density was observed.
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