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NADP+- Based Enzyme Assay for D-isocitrate

 

作者: Ashutosh Sharma,   NigelS.M. Quantrill,   KimR. Rogers,  

 

期刊: Analytical Letters  （Taylor Available online 1997）
卷期: Volume 30, issue 9  

页码: 1667-1674

 

ISSN:0003-2719

 

年代: 1997

 

DOI:10.1080/00032719708001685

 

出版商: Taylor & Francis Group

 

关键词: enzyme assay;fluorescence quenching;NADPH;isocitrate;isocitrate dehydrogenase

 

数据来源: Taylor

 

摘要:

A novel procedure for the measurement of D-isocitrate is described that utilizes the fluorescence quenching of thionine by the reduced form of the cofactor NADPH, which results from the isocitrate dehydrogenase-catalyzed conversion of D-isocitrate to 2-oxoglutarate. The procedure is termed substrate induced-quenching (SIQ) assay and results in a fluorescence-quenching constant (KSIQ) of 4443 M−1obtained from data collected over the range of 0–500 μM isocitrate.

 

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