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Simplified Method for the Isolation and Analysis of Ethynyl Steroids in Urine

 

作者: S.H. G. Andersson,   M. Axelson,   B.-L. Sahlberg,   J. Sjövall,  

 

期刊: Analytical Letters  （Taylor Available online 1981）
卷期: Volume 14, issue 10  

页码: 783-790

 

ISSN:0003-2719

 

年代: 1981

 

DOI:10.1080/00032718108055482

 

出版商: Taylor & Francis Group

 

关键词: Ethynyl steroids;liquid-solid extraction;ligand exchange chromatography

 

数据来源: Taylor

 

摘要:

A simplified procedure for selective isolation of ethynyl steroids from urine is described. Urinary steroids are extracted with Sep-PakRC18and subjected to enzyme hydrolysis. The liberated steroids are extracted with Lipidex 1000 and the eluate from this column is passed through a bed of sulfohydroxypropyl Sephadex LH-20, partly converted into silver form by a wash with aqueous silver nitrate. Ethynyl steroids are eluted with a solution of acetylene in methanol.

 

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