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A New Human Highly Tumorigenic Neuroblastoma Cell Line with Undetectable Expression of N-myc1

 

作者: P CORNAGLIA-FERRARIS,   M PONZONI,   P MONTALDO,   G L MARIOTTINI,   E DONTI,   D DI MARTINO,   G P TONINI,  

 

期刊: Pediatric Research  (OVID Available online 1990)
卷期: Volume 27, issue 1  

页码: 1-6

 

ISSN:0031-3998

 

年代: 1990

 

出版商: OVID

 

关键词: NB, neuroblastoma;GI-ME-N;Gaslini Institute;patient initials;neuroblastoma;PNET, peripheral neuroectodermal tumor;BM, bone marrow;s.c, subcutaneous

 

数据来源: OVID

 

摘要:

A peculiar human cell line (GI-ME-N) derived from the metastatic bone marrow of a 2-yr-old patient with stage IV neuroblastoma (NB) was extensively characterized. Cell-type-specific markers, tumorigenicity in nude mice, morphology, cytogenetics, and amplification/expression of the N-myc gene were evaluated. All metaphases presented the typical 1p deletion. Surface markers specific for NB cells, vimentin, and neurofilament proteins were all clearly detectable with immunofluorescence and/or western blot procedures. Moreover, it was found that GI-ME-N cells did not express N-myc oncogene or HLA class 1 antigens, and were not classified as peripheral neuroectodermal tumor cells. However, extremely short latency and survival times, comparable to peripheral neuroectodermal tumor cells, were observed in nude mice grafted with GI-ME-N. In addition, no correlations were. observed in tumorigenicity of N-myc amplified (IMR32)versusunamplified (SK-N-SH GI-ME-N) human NB cell lines in nude mice. We conclude that N-myc amplification/ expression do not correlate with the aggressiveness of human NB in athymic animals, which is not always explained by the peripheral neuroectodermal tumor cell nature of the malignant cells, either.

 

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