The South African bread wheat cultivar Tugela has consistently displayed effective field resistance to leaf rust, caused byPuccinia reconditaRob. ex Desm. f. sp.tritici.Based on its pedigree and adult plant reaction to leaf rust, it has been assumed that Tugela contains theLr34gene for resistance toP. reconditaf. sp.tritici.Evaluation of wheat seedings at 10°C and 20°C suggested that Tugela has two genes for resistance, one of which is only expressed at 10°C. Tugela primary leaf infection types at 10°C and measurement of latent period, uredinium density and uredinium size on flag leaves strongly resembled those on RL6058 (Thatcher/Lr 34). To determine the number ofLrgenes and confirm the presence ofLr34, Tugela was crossed with Thatcher (leaf rust susceptible) and RL6058. F2and F3populations from each cross were tested as seedlings for segregation of resistance to pathotype UVPrt3 at 10°C. Two dominant genes were indicated by the segregation ratios of Tugela×Thatcher F2plants (χ215:1= 0.56) and F3families (χ27:8:1= 0.67). The presence ofLr34was confirmed in all 590 Tugela×RL6058 F2seedlings tested. Tugela×Thatcher F2plants, inoculated with pathotype UVPrt9 during flag leaf and ear emergence stages of growth, indicated segregation of a single gene (χ23:1= 0.19) for resistance when tested at low glasshouse temperature. All Tugela×RL6058 adult F2plants were resistant in this environment. Field tests with F3families from both crosses confirmed glasshouse results. This study indicated that Tugela containsLr34and an unidentifiedLrgene.