The inactivation of rhodanese by nitrite and inhibition by other anions in vitro
作者:
Keith Alexander,
Lawrence R. Procell,
Stephen D. Kirby,
Steven I. Baskin,
期刊:
Journal of Biochemical Toxicology
(WILEY Available online 1989)
卷期:
Volume 4,
issue 1
页码: 29-33
ISSN:0887-2082
年代: 1989
DOI:10.1002/jbt.2570040106
出版商: Wiley Subscription Services, Inc., A Wiley Company
关键词: Rhodanese;Nitrite;Cyanide;Thiosulfate;Anion Inhibition
数据来源: WILEY
摘要:
AbstractCyanide detoxification in mammals occurs, in part, by sulfur transfer by rhodanese to form the less toxic thiocyanate. Thiosulfate and nitrite are often used in combination for the treatment of cyanide intoxication. This report shows that nitrite can inhibit the rate of sulfur transfer by rhodanese in vitro. Nitrate, chloride, sulfate, and acetate were also examined as inhibitors. Inhibition by nitrite appeared to be more complex than for the other anions tested. Closer examination showed that nitrite can inactivate the sulfur‐free rhodanese. Our observation leads to the suggestion that, in vivo, either rhodanese is maintained in its more stable sulfur‐substituted form or cellular compartmentalization prevents inactivation by nitr
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