At least 120 species of Coleoptera were recovered from poultry manure in narrow, widespan, and high-rise types of caged-layer poultry houses in the Mountains, Piedmont, and Coastal Plain regions of North Carolina.Carcinops pumilio(Erichson) andAlphitobius diaperinus(Panzer) were consistently the most abundant species in all 3 regions, followed byGnathoncus nanus(Scriba) and the Aleocharinae (Staphylinidae). According to 5 indices of diversity, the Piedmont ranked 1st in overall diversity, species richness and equitability, followed by the Coastal Plain, and thirdly, by the Mountains.Narrow and high/rise house types were compared for adult and larvalC. pumilioandA. diaperinus. The larval population peak forA. diaperinusoccurred significantly earlier in the high-rise than in the narrow houses, however, the narrow houses had significantly higher beetle diversity.A qualitative examination of overwintering forms showed that most species overwintered primarily, but not exclusively, as adults.