Myxosoma cerebralis: Isolation and Concentration from Fish Skeletal Elements — Sequential Enzymatic Digestions and Purification by Differential Centrifugation
作者:
Maria E. Markiw,
Ken Wolf,
期刊:
Journal of the Fisheries Board of Canada
(NRC Available online 1974)
卷期:
Volume 31,
issue 1
页码: 15-20
ISSN:0706-652X
年代: 1974
DOI:10.1139/f74-003
出版商: NRC Research Press
数据来源: NRC
摘要:
An effective sequential procedure for recovery ofMyxosoma cerebralisspores from infected trout was developed, and quantification of spores was carried out at each step of release and concentration. Methods are described for fresh and frozen material. Effective concentration of from 1100- to 9000-fold and an estimated efficiency of recovery of about 80% has been achieved. Tabular and graphic data are presented with recommendations for diagnostic applications. The immediate applications of these procedures are in implementing more effective detection and in preparing antigen for the immunologic studies that should provide the most sensitive detection.
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