Quantifying HIV‐1 Proviral DNA Using the Polymerase Chain Reaction on Cerebrospinal Fluid and Blood of Seropositive Individuals With and Without Neurologic Abnormalities
作者:
Peter Schmid,
Andrew Conrad,
Karl Syndulko,
Elyse Singer,
Daniel Handley,
Ximing Li,
Gongming Tao,
Bridget Fahy-Chandon,
Wallace Tourtellotte,
期刊:
Journal of Acquired Immune Deficiency Syndromes
(OVID Available online 1994)
卷期:
Volume 7,
issue 8
页码: 777-788
ISSN:0894-9255
年代: 1994
出版商: OVID
关键词: HIV-1;Neurologic disease;Blood;Cerebrospinal fluid;CD4+cells;Quantified DNA PCR;Proviral load.
数据来源: OVID
摘要:
SummaryTo quantify the number of human immunodeficiency virus type 1 (HIV-1) proviral copies per 1,000 CD4+cells in cerebrospinal fluid (CSF) and blood in relationship to stage of infection and HIV-1 neurologic disease (HND), 87 HIV-1 seropositive men without CNS opportunistic infections, tumors, or neurosyphilis, 9 high-risk, and 14 not-at-risk seronegative controls underwent a structured interview, and physical and neurologic examination followed by blood and CSF collection. A custom-designed, fully automated polymerase chain reaction (PCR) system performed amplification with use of two HIV-1gagprimer pairs, Southern blotting, and hybridization with product-specific probes. Image analysis was used to quantify band intensities relative to a dilution series. Eighty-one of 87 (93%) seropositive patients, 1 of 9 high-risk patients, (11%) and none of 14 seronegative controls had PCR-detectable HIV-1 in their blood. Fifty-seven of 63 (90%) seropositive patients, 2 of 5 (40%) high-risk seronegative patients, and none of 14 controls had HIV-1 in their CSF. The proviral load in seropositive patients, all stages, was significantly greater in CSF than blood [median 25 vs. 0.6 copies/1,000 CD4+cells (p = 0.0001)]. The median proviral load in blood was 0.09 copies/1,000 CD4+cells in seropositive, asymptomatic subjects, 10.7 in patients with AIDS, and 1.4 in patients with AIDS-related complex (p = 0.0281). CSF proviral load was greater in seropositive patients with HND than those without HND, median 43.5 vs. 17.6 copies/1,000 CD4+cells (p = 0.0614). Proviral load was greater in the blood and CSF of subjects with more advanced systemic disease and HND. There was a substantial penetration of HIV-1 into the CNS/CSF in both systemically and neurologically asymptomatic HIV-1 disease.
点击下载:
PDF
(962KB)
返 回